A. Personal Statement
The field of Prof Tell prevailing interests is the study of molecular mechanisms of gene expression particularly in the field of redox signalling and cell oxidative stress. Now, he is focusing on some aspects linking gene expression and DNA repair and its relevance in molecular oncology and cancer.
In particular, from 1998, he contributed to the understanding of the molecular mechanisms involving the main mammalian Apurinic/Apyrimidinic Endonuclease APE1 in coordinating cellular responses to oxidative stress in different cell models. His background includes molecular and cellular biology as well as biochemistry techniques to characterize the relationship between structure and function of proteins involved in gene expression and DNA repair. He coordinated several research projects granted from Telethon, AIRC, PRIN, ASI and worked as a Referee for 20 different International Journals (Oncogene, Nucleic Acids Research, Proteomics, Cancer Research, etc).
B. Positions and Honors
Positions and Employment
- From November 2005-present, he is Associate Professor of Molecular Biology, at the Department of Biomedical Sciences and Technologies and from January 2011 at the Department of Medical and Biological Sciences, School of Medicine, University of Udine, Udine, Italy;
- July-September 2011, Visiting Research Scholar in the lab of Prof. Bruce Demple. Department of Pharmacological Sciences, Stony Brook University, Stony Brook, NY, USA.
- July 2009, Visiting Professor in the lab of Prof. Pablo Radicella. CEA, Institut de Radiobiologie Cellulaire et Moléculaire, UMR217 CNRS, F-92265 Fontenay-aux-Roses, France.
- June-August 2006, Visiting Professor in the lab of Prof. Sankar Mitra.
School of Medicine-Sealy Center For Molecular Science And Department Of Human Biological Chemistry And Genetics. University of Texas, Galveston, TX, USA.
- September 2003-October 2005, Assistant Professor of Molecular Biology, Department of Biomedical Sciences and Technologies, School of Medicine, University of Udine, Italy;
- March 2000-September 2003, Assistant Professor of Molecular Biology, Department of Biochemistry, Biophysics and Macromolecular Chemistry, School of Medicine, University of Trieste, Italy;
- 1996, visiting scientist in the lab of Dr. David Segal, Experimental Immunology Branch, Division of Basic Sciences, NCI, NIH, Bethesda (MD) USA;
- Staff fellow, 1994-March 2001, Department of Biomedical Sciences and Technologies, School of Medicine, University of Udine, Italy;
1998 – 1999 Deputy Associate Professor, General Pathology, Department of Biomedical Sciences and Technologies, Udine University Medical School, Udine – Italy
1997 – 1999 Teaching assistant, Immunology, Department of Biomedical Sciences and Technologies, Udine University Medical School, Udine – Italy
1997 – 1998 Teaching assistant, Biochemical and Molecular Gene Expression Techniques, Department of Biomedical Sciences and Technologies, Udine University Medical School, Udine – Italy
2008-2012 Consultant, Ministry of Science (Georgia)
2008 Invited Speaker at Anticancer Research Congress in Kos, Greece;
2009 Invited Speaker at US/EU DNA repair meeting in Galveston, TX-USA.
2010 Invited Speaker at INBB meeting in Rome, Italy.
2011 UICC Yamagiwa-Yoshida Memorial International Cancer Study Grant funded by the Kyowa Hakko Kogyo Company Ltd., Tokyo and the Japan National Committee for UICC
2011 Consultant, Ministry of University and Research (Italy)
The field of prevailing interest is the study of molecular mechanisms of gene expression particularly in the field of redox signalling and cell oxidative stress. In the last years he devoted himself to the use of Proteomics approaches to the study of these topics and on the role of the protein APE1 in coordinating cellular responses to oxidative stress in different models. He coordinated several research projects granted from Telethon, AIRC, PRIN, ASI and worked as a Referee for 20 different International Journals.
Prof. G. Tell authored more than 100 publications in international peer reviewed journals and more than 70 international congress communications concerning control of gene expression during response to oxidative stress. Total Impact Factor >500. The value of citation index (h-index) according to ISI Web of Knowledge is of 24 with a mean citation value of 11.52.
First author publications: 23
Last author publications: 37
Total publications: 122
C. Selected Peer-reviewed Publications (20 out of 122 total)
1. Tell, G., Pellizzari, L., Cimarosti, D., Pucillo, C. and Damante, G. “Ref-1 controls Pax-8 DNA-binding activity”. (1998) Biochem. Biophys. Res. Commun. 252, 178-183.
2. Tell, G., Zecca, A., Cimarosti, D., Colombatti, A., Kelley, M., Damante, G. and Pucillo, C. “An ‘environment to nucleus’ signaling system operates in B lymphocytes: redox status of Ref-1 modulates BSAP/Pax-5 activation”. (2000) Nucleic Acids Research. 28, 1099-1105.
3. Tell, G., Pellizzari, L., Pucillo, C., Puglisi, F., Cesselli, D., Kelley, M.R., Di Loreto, C. and Damante, G. “TSH controls Ref-1 nuclear translocation in thyroid cells". (2000) J. Mol. Endocrinol. 24, 383-390.
4. Tell, G., Crivellato, E., Pines, A., Paron, I., Pucillo, C., Manzini, G., Bandiera, A., Kelley, M.R., Di Loreto, C. and Damante, G. “Mitochondrial localization of APE/Ref-1 in thyroid cells”. (2001) Mutat. Res. 485, 143-152.
5. Tell, G., Pines, A., Paron, I., D’Elia, A., Bisca, A., Kelley, M.R., Manzini, G. and Damante, G. Ref-1 regulates the activity of thyroid transcription factor 1 by controlling the redox state of the N transcriptional activation domain. (2002) J. Biol. Chem. 277, 14564-14574.
6. Tell, G., Pines, A., Pandolfi, M., D'Elia, A., Donnini, D., Lonigro, R., Manzini, G., Russo, D., Di Loreto, C. and Damante, G. APE/Ref-1 is controlled by both redox and cAMP-dependent mechanisms in rat thyroid cells. (2002) Horm. Metab. Res. 34, 303-310
7. Frossi, B., Tell, G., Spessotto, P., Colombatti, A., Vitale, G. and Pucillo, C. H2O2 induces translocation of APE/Ref-1 to mitochondria in the Raji B-cell line. (2002) J. Cell. Physiol. 193, 180-618.
8. Tell, G., Damante, G., Caldwell, D., and Kelley, M.R. The intracellular localization of APE1/Ref-1: more than a passive phenomenon? (2005) Antiox. Redox. Signal 7, 367-84.
9. Pines A, Perrone L, Bivi N, Romanello M, Damante G, Gulisano M, Kelley MR,Quadrifoglio F, Tell G. Activation of APE1/Ref-1 is dependent on reactive oxygen species generated after purinergic receptor stimulation by ATP. Nucleic Acids Res. (2005) 33, 4379-4394.
10. Di Maso V, Avellini C, Croce LS, Rosso N, Quadrifoglio F, Cesaratto L,Codarin E, Bedogni G, Beltrami CA, Tell G, Tiribelli C. Subcellular Localization of APE1/Ref-1 in Human Hepatocellular Carcinoma: Possible Prognostic Significance. Mol Med. (2007) 13:89-96.
11. Cesaratto L, Calligaris SD, Vascotto C, Deganuto M, Bellarosa C, Quadrifoglio F, Ostrow JD, Tiribelli C, Tell G. Bilirubin-induced cell toxicity involves PTEN activation through an APE1/Ref-1-dependent pathway. J Mol Med. (2007) 85:1099-112.
12. Tell G., Quadrifoglio F., Tiribelli C., Kelley MR. The many functions of APE1/Ref-1: not only a DNA-repair enzyme. Antioxid. Redox. Signal. (2009), 11(3): 601-620. doi:10.1089/ars.2008.2194.
13.Vascotto C., Cesaratto L., Zeef L.A.H., Deganuto M., D’Ambrosio C., Scaloni A., Romanello M., Damante G., Taglialatela G., Delneri D., Kelley M.R., Mitra S., Quadrifoglio F., Tell G. Genome-wide analysis and proteomic studies reveal APE1/Ref-1 multifunctional role in mammalian cells. Proteomics (2009), 9:1058-1074.
14. Vascotto C, Fantini D, Romanello M, Cesaratto L, Deganuto M, Leonardi A,Radicella JP, Kelley MR, D'Ambrosio C, Scaloni A, Quadrifoglio F, Tell G. APE1/Ref-1 interacts with NPM1 within nucleoli and plays a role in the rRNA quality control process. Mol. Cell Biol. (2009), 29(7):1834-54.
15. Tell G, Wilson DM 3rd, Lee CH. Intrusion of a DNA repair protein in the RNome world: is this the beginning of a new era? Mol Cell Biol. 2010 Jan;30(2):366-71.
16. Tell G, Wilson DM 3rd. Targeting DNA repair proteins for cancer treatment. Cell Mol Life Sci. (2010) 67(21):3569-72.
17. Tell G, Fantini D, Quadrifoglio F. Understanding different functions of mammalian AP endonuclease (APE1) as a promising tool for cancer treatment. Cell Mol Life Sci. (2010) 67(21):3589-608.
18. Fantini D, Vascotto C, Marasco D, D'Ambrosio C, Romanello M, Vitagliano L, Pedone C, Poletto M, Cesaratto L, Quadrifoglio F, Scaloni A, Radicella JP, Tell G. Critical lysine residues within the overlooked N-terminal domain of human APE1 regulate its biological functions. Nucleic Acids Res. (2010) 38(22):8239-56.
19. Vascotto C., Bisetto E., Li M., Zeef LAH, D’Ambrosio C, Domenis R, Comelli M, Delneri D, Scaloni A, Altieri F, Mavelli I, Quadrifoglio F, Kelley MR, Tell G. Knock-in reconstitution studies reveal an unexpected role of Cys65 in regulating APE1/Ref-1 subcellular trafficking and function. Mol Biol of The Cell (2011) 22(20):3887-901.
20. Lirussi L, Antoniali G, Vascotto C, D'Ambrosio C, Poletto M, Romanello M, Marasco D, Leone M, Quadrifoglio F, Bhakat K, Scaloni A, Tell G. Nucleolar accumulation of APE1 depends on charged Lysine residues that undergo acetylation upon genotoxic stress and modulate its BER activity in cells. Mol Biol Cell. 2012 Aug 23. [Epub ahead of print] PubMed PMID: 22918947.
D. Research Support
Prof Tell’s salary and laboratory resources (including personnel, space and supplies) are covered by the University of Udine, Italy.
Ongoing Research Support
2010-13 Research grant AIRC #IG10269 (Understanding the functional regulation of APE1 for development of new specific inhibitors)
2010-12 Telethon, Grant # GGP10051B (New diagnostic and therapeutic approaches for the Crigler−Najjar Syndrome Type I)
2010-12 Research grant PRIN2008 #2008CCPKRP (Molecular networks involving APE1 and role of post-translational modifications in fine-tuning the APE1 different functions for development of new drugs for cancer treatment)
2009-2010 ITALY/FRANCE ‘Galileo’ exchange grant from the Università Italo-Francese.
2008-11 Grant FIRB-National Proteomics Network RBRN07BMCT (Italian Human ProteomeNet)
2008-10 EU/USA Exchange Grant by Ministry from Foreign Affairs: Role of Ape1 in Neurotoxicity of Cancer Treatments